Figure 1.

Overview of viral vector construction, experimental plan for injections, and behavioral analyses. (a) Schematic depiction of the AAV–FMRP vector construct. The single-stranded AAV viral vector contained the human SYN promoter and ITR elements from AAV serotype 2 and packaged in serotype 9 capsids. A woodchuck hepatitis post-transcriptional regulatory element was inserted downstream of the mouse Fmr1 cDNA to induce elevation of transcripts. (b) Timelines for the short and long arms of the study for mice injected with PBS or viral vector on PND 5. The total number of mice (N) injected for each treatment group is indicated.