Figure 4.

Rapid induction of IFNγ and IL-4 by immunisation with I-αGC-LM. BALB/c mice were injected i.p. with inert vehicle, LM, I-αGC-LM or free αGC. Sera were assayed at the indicated times for IFNγ by capture ELISA (A). Values of IFNγ for vehicle-treated mice were below the limit of detection (<0.1 ng ml⁻¹). Sera obtained from mice (N=3 per group) at 2 h after treatment as in A were assayed for IL-4 by capture ELISA (B). Graphs are a representative of three experiments with N=3 mice per group. Mean and s.d. are shown, and significant elevations of IFNγ above background levels are indicated by asterisks. *P<0.05, **P<0.01, ***P<0.001; ANOVA with Sidak post-test for multiple comparisons.