Skip to main content
. 2014 Oct 14;111(10):1945–1954. doi: 10.1038/bjc.2014.486

Figure 6.

Figure 6

Enhancement of CD8+ and NK cell responses following therapeutic immunisation with I-αGC-LM-Mb. BALB/c mice were challenged with 4T1 tumour cells and immunised therapeutically i.p. with saline, LM-Mb, I-αGC-LM-Mb or αGC as described in Figure 1. Spleens were collected on day 18 and analysed for IFNγ-producing cells by ELISPOT. Measurement of IFNγ spot-forming cells (SFC) determined by ELISPOT following restimulation in vitro with Mage-b, either without (hatched bars) or with (solid bars) depletion of CD8α+ cells (A). White blood cells were analysed directly ex vivo by flow cytometry for intracellular IFNγ+ cells, gating on CD8α+ lymphocytes (B). Measurement of IFNγ SFC in the spleen was determined by ELISPOT following restimulation in vitro with LM, either without (hatched bars) or with (solid bars) depletion of DX5+ NK cells (C). These experiments were repeated three times with N=5 mice per group. Means and s.e. are shown. **P<0.01, ***P<0.001 (Mann–Whitney test).