Analysis of osteomimetic scaffolds. (A) SEM image of ECM covering surface of PLGA scaffold, scale bar 10 μm and magnification 2000×; (B) Alizarin Red S staining of calcium deposited by hOBs on scaffold surface, scale bar 200 μm and magnification 10×; (C) Collagen II staining of ECM confirms ECM composition, scale bar 50 μm and magnification 20×; (D) ALP staining of ECM covering surface of PLGA scaffold, scale bar 500 μm and magnification 4×. ALP, alkaline phosphatase; ECM, extracellular matrix; SEM, scanning electron microscopy. Color images available online at www.liebertpub.com/tec