FIG. 6.

Calcium deposition and gene expression of differentiated hESCs on 2D culture plates, Geltrex-coated PLGA, and osteomimetic PLGA. * and ^ represent significant difference in calcium content and gene expression by cells on osteomimetic scaffolds compared with Geltrex-coated PLGA and 2D tissue culture plates at significance levels of p<0.05 and 0.5, respectively. (A) Calcium quantification for each group represented as microgram/substrate where the substrate is the scaffold for the osteomimetic PLGA and geltrex-coated PLGA groups, and the 2D well surface for the TCPS group; (B) Relative RUNX2 gene expression (n=2); (C) Relative BGLAP gene expression (n=2). All values were normalized to GAPDH. 2D, two-dimensional; TCPS, tissue culture polystyrene.