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. 2014 Oct 1;12(8):481–496. doi: 10.1089/adt.2014.603

Fig. 6.

Fig. 6.

DMSO tolerance. (A) DMSO (1% v/v) has no apparent effect upon fluorescence intensity measurements for PP5C reaction progress curves. PP5C (350 pM) was incubated as described in “Materials and Methods” section with 50 μM OMFP in buffer with (▼) and without (▲) the presence of 1% DMSO. Fluorescence intensity measurements were conducted on reactions in each well (40 replicates for each condition) every 30 s over 5 min (485-nm excitation/525-nm emission) with the M5. Reaction progress curves are plotted together with GraphPad Prism and are almost perfectly superimposable. (B) DMSO (1% v/v) has no apparent effect upon fluorescence intensity measurements for PP1α reaction progress curves. PP1α (3 nM) was incubated as described in “Materials and Methods” section with 100 μM OMFP in buffer with (▼) and without (▲) the presence of 1% DMSO. Fluorescence intensity measurements were conducted on reactions in each well (40 replicates for each condition) every 30 s over 5 min (485-nm excitation/525-nm emission) with the M5. Reaction progress curves are plotted together with GraphPad Prism and are almost perfectly superimposable.