FIG. 5.

Representative sections and analysis of (A) Hairless and (D) Plunc mRNA expression in the ependymal layer and ARC of Sprague Dawley rats that were untreated (black bar, control, n=9) and compared with animals rendered hypothyroid over 21 days as in Figure 1, before being injected with T3 (MMI-T3, n=8, white bars) or saline (MMI-sal, n=8, gray bars). Expression was normalized to the control group. (B) and (E) show representative sections and analysis of Hairless and Plunc mRNA respectively in the ependymal layer of untreated rats that were fed ad libitum (black bar, ad lib, n=8), fasted for 48 hours (light gray bar, fasted, n=8) or fasted for 48 hours and then re-fed for 24 hours (dark gray bar, re-fed, n=8). (C) Hairless expression was analyzed in the ependymal layer of Siberian hamsters that were kept in LD (16:8 light:dark, black bar) or SD for 14 weeks (8:16 light:dark, light gray bar). No Plunc expression was present in the ependymal layer of Siberian hamsters. Data were normalized to LD hamsters. Data are presented as mean±SEM. Bars with different letters are significantly different.