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Calcification of pericardium samples from in vivo subcutaneous model: histological analysis. Samples explanted at 3 and 6 weeks were fixed and prepared for histological analysis using Von Kossa stains. The dark black region indicates calcium phosphate deposits. This region is seen significantly darker in GA-treated samples after 3 and 6 weeks of implantation and not observed in PPF reinforced or untreated samples. Scale bars represent 100 μm.
