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Comparison of percentage of cells in different culture conditions revealed by immunofluorescent labeling and patch clamping experiments
| TG Neuron culture condition | Immunofluorescence | Patch clamp recording | ||||
|---|---|---|---|---|---|---|
| P2X3 only | P2X2 + P2X2/3 | Transient current | Sustained current | |||
| P2X2 only | P2X2 + P2X3 | total | P2X3 only | P2X2 + P2X2/3 | ||
| Normal | 14% | 17% | 19% | 36% | 17% | 34% |
| Co-culture | 11% | 17% | 33% | 50% | 14% | 44% |
| Co-culture + Anti-NGF | 11% | 21% | 20% | 41% | 16% | 49% |
