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. 2014 Jun 13;2:72. doi: 10.1186/2051-5960-2-72

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© Liu et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cell identification using WSA automated and manual. (A) In some cases, WSA automated quantified NeuN immunopositive cells close to the grey/white matter demarcation (white arrow); some of these cells might be neurons in the cortical layer VI. (B) In contrast, because the user drew the white matter with a set distance away (0.5 mm) from the grey/white matter demarcation in WSA manual, we could be confident that the counted cells were in the white matter.