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Verification of sRNAs results by Northern Blot. Northern blot analysis of csrB and crsC sRNAs expression under aerobic/anaerobic conditions. 10 μg of total RNA obtained from S. flexneri M90T wild‒type strain and its isogenic Δfnr mutant grown under aerobic and anaerobic conditions until OD600 = 0.2 were separated in 1,25% MOPS‒agarose gels, transferred to membranes and detected using probes specific for the sense strand.
