FIG. 4.

(A) Macroscopic image of tissues engineered on transwell inserts at 5% pO₂ or 20% pO₂. Scale bar=5 mm. (B) DNA, GAG, collagen, GAG/DNA, collagen/DNA, GAG (% wet weight [ww]), collagen (% ww) content of tissues engineered at 5% pO₂ or 20% pO₂. (C) Young's modulus of tissues engineered at 5% pO₂ or 20% pO_2. (D) Histological staining of engineered tissue for Alcian Blue, Picrosirius Red, and immunostaining for type II collagen. Scale bar in lower magnification image=500 μm. Scale bar in higher magnification image=100 μm. *indicates p<0.05. Color images available online at www.liebertpub.com/tea