(A) Macroscopic image of tissues engineered on transwell inserts in the presence or absence of fetal bovine serum (FBS). Scale bar=5 mm. (B) Histological staining of engineered tissue for Alcian Blue and Picrosirius Red. Scale bar=500 μm. (C) DNA, GAG, collagen, GAG/DNA, collagen/DNA, GAG (% ww), collagen (% ww) content of tissues engineered in the presence or absence of fetal bovine serum (FBS). *indicates p<0.05. Color images available online at www.liebertpub.com/tea