FIG. 2.

The cellular composition of scaffold channels was identified with antibodies to glial fibrillary acid protein (GFAP) (red) and the S-100 antigen (green) in (A) Matrigel and in (B) Schwann cell channels at 20× magnification and (C) Schwann cell channels at 60× magnification. GFAP staining uniformly demarcated the channel periphery, while S-100 and GFAP staining colocalized in discrete cell populations within the core region. (D) eGFP-MSCs cellularity was further visualized at 40× magnification. (E) GFAP (red) peripheral staining, and GFAP/S-100 (magenta) colocalization in the channel core was also a feature of eGFP-MSC (green) channels, seen here at 40× magnification. Color images available online at www.liebertpub.com/tea