FIG. 4.
(A–C) Axonal neurofilament protein was visualized with antibody staining and the 3,3′-diaminobenzidine chromogen (brown) in Matrigel, Schwann Cell, and eGFP-MSC channels (10× magnification). (D–F) Collagen IV antibody staining (red) identified blood vessels in Matrigel, Schwann cell, and eGFP-MSC (green) channels counterstained with DAPI nuclear staining (blue) (40× magnification). Matrigel and Schwann cell channel blood vessels were of small diameter and were thin walled. Blood vessels in eGFP-MSC channels were of larger diameter. eGFP-MSCs were frequently seen to be aligned along the vessel perimeter (F). Color images available online at www.liebertpub.com/tea