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. 2013 Sep 24;101(1):39–47. doi: 10.1093/cvr/cvt222

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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2013. For permissions please email: journals.permissions@oup.com.

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β2-Spectrin is prominent in the developing heart. (A) X-gal staining of a whole-mount Spnb2^+/tm1a embryo (right) at E9.5 revealed the expression of β2-spectrin. (B) X-gal staining of a frozen section of a Spnb2^+/tm1a embryo at E16.5. H, heart; L, liver; Lu, lung; S, spinal cord. (C) At E16.5, expression of β2-spectrin was detected in both compact and trabecular areas of the heart. (D) Normal heart from a wild-type embryo at E12.5 was stained with the antibody against β2-spectrin. The right panels represent magnifications of the boxed area. Scale bars, 2 mm (B); 100 μm (C); 200 μm (D).