Table 1.
Primers used for PCR to detect Shigella resistance and virulence genes
| Primer | Sequence (5′-3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
| ESBL genes | |||
|
CTX-M-1 group |
F-GGCCCATGGTTAAAAAATCACTGC |
891 |
[3] |
| |
R-CCGTTTCCGCTATTACAAACCGTTG |
|
|
|
CTX-M-2 group |
F-CTCAGAGCATTCGCCGCTCA |
843 |
|
| |
R-CCGCCGCAGCCAGAATATCC |
|
|
|
CTX-M-8 group |
F-ATGATGAGACATCGCGTTAAC |
864 |
|
| |
R-CGGTGACGATTTTCGCGGCAG |
|
|
|
CTX-M-9 group |
F-GTGACAAAGAGAGTGCAACGG |
856 |
|
| |
R-ATGATTCTCGCCGCTGAAGCC |
|
|
|
OXA group |
F-ACACAATACATATCAACTTCGC |
813 |
|
| |
R-AGTGTGTTTAGAATGGTGATC |
|
|
|
SHV group |
F-GCCTTTTCGGCCTTCACTCAAG |
989 |
|
| |
R-TTAGCGTTGCCAGTGCTCGATCA |
|
|
|
TEM group |
F-ATAAAATTCTTGAAGACGAAA |
1076 |
|
| |
R-GACAGTTACCAATGCTTAATC |
|
|
| AmpC β-lactamase genes | |||
|
MOX group |
F-GCTGCTCAAGGAGCACAGGAT |
520 |
[14] |
| |
R-CACATTGACATAGGTGTGGTGC |
|
|
|
CIT group |
F-TGGCCAGAACTGACAGGCAAA |
462 |
|
| |
R-TTTCTCCTGAACGTGGCTGGC |
|
|
|
DHA group |
F-AACTTTCACAGGTGTGCTGGGT |
405 |
|
| |
R-CCGTACGCATACTGGCTTTGC |
|
|
|
ACC group |
F- AACAGCCTCAGCAGCCGGTTA |
346 |
|
| |
R- TTCGCCGCA ATCATCCCTAGC |
|
|
|
EBC group |
F- TCGGTAAAGCCGATGTTGCGG |
302 |
|
| |
R- CTTCCACTGCGGCTGCCAGTT |
|
|
|
FOX group |
F- AACATGGGGTATCAGGGAGATG |
190 |
|
| |
R- CAA AGCGCGTAACCGGATTGG |
|
|
| Virulence gene | |||
|
ial |
F-CTGGATGGTATGGTGAGG |
320 |
[12] |
| |
R-GGAGGCCAACAATTATTTCC |
|
|
|
ipaH |
F-TGGAAAAACTCAGTGCCTCT |
423 |
|
| |
R-CCAGTCCGTAAATTCATTCT |
|
|
|
setlA |
F-TCACGCTACCATCAAAGA |
309 |
|
| |
R-TATCCCCCTTTGGTGGTA |
|
|
|
setlB |
F-GTGAACCTGCTGCCGATATC |
147 |
|
| |
R-ATTTGTGGATAAAAATGACG |
|
|
|
sen |
F-ATGTGCCTGCTATTATTTAT |
799 |
|
| |
R-CATAATAATAAGCGGTCAGC |
|
|
|
virA |
F-CTGCATTCTGGCAATCTCTTCACATC |
215 |
[13] |
| R-TGATGAGCTAACTTCGTAAGCCCTCC | |||