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. 2014 Jun 4;2:60. doi: 10.1186/2051-5960-2-60

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© Menard et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Intrinsic cardiac neuron cultures. Panels A and B are representative of normal neuronal cells in culture identified via phase contrast microscopy. Panel A demonstrates STZ-diabetic rat neurons at 48 hours in culture. Panel B demonstrates age-matched control rat neurons at 48 hours in culture. Several different neuronal types were isolated, including large pseudounipolar appearing cells similar to dorsal root ganglion cells (thin arrows), as well as bi- (arrowheads) and multi-polar (thick arrows) cells indicative of efferent or interneuronal cell types.