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. 2014 Jun 4;2:60. doi: 10.1186/2051-5960-2-60

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© Menard et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Intensity of 4-HNE adduct staining. Panels A and B are the graphical representation of the fluorescence intensity recorded for 4-HNE adduct staining vs. the total 4-HNE staining neurite area for both 3- and 6-month STZ-diabetic and age-matched control rat neuronal cultures. An asterisk (*) indicates statistically significant data. Error bars indicate the mean ± the S.E.M. At 3 months (panel A), there was no statistical significance in 4-HNE adduct staining between diabetic and control neurons (p = 0.5755). At 6 months (Panel B), there was a significant difference observed, with the diabetic neurons displaying a 4-HNE adduct staining intensity per total 4-HNE staining area that was 2.22X that seen in the controls (p = <0.0001).