Table 2.
Scheme of in vitro stimulation of mononuclear cells.
| Cells (107 cells/mL) |
Stimulation | Cultivation X-Vivo medium |
|
|---|---|---|---|
| PWM | 100 µL | 100 µL PWM | 800 µL |
| PWM + ConA | 100 µL | 100 µL PWM + 100 µL ConA | 700 µL |
| HSP70 | 100 µL | 100 µL | 800 µL |
| A.a. | 100 µL | 100 µL A.a. | 800 µL |
| P.g. | 100 µL | 100 µL P.g. | 800 µL |
| T.f. | 100 µL | 100 µL T.f. | 800 µL |
| Unstim. | 100 µL | 900 µL |
PWM: Pokeweed mitogen, ConA: Concanavalin A, HSP70: Heat Shock Protein 70, A.a.: Aggregatibacter actinomycetemcomitans, P.g.: Porphyromonas gingivalis, T.f.: Tannerella forsythia, and unstim.: without stimulation.