(a).
| Locus | Periodontitis patients | Healthy controls | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IL-4-590 | IL-4VNTR | IL-4-590 | IL-4VNTR | |||||||||||||
| Polymorphism | CC | CT + TT | 11 | 12 + 22 | CC | CT + TT | 11 | 12 + 22 | ||||||||
| (Number of patients) | (30) | (16 + 1) | (28) | (17 + 1) | (8) | (5 + 2) | (10) | (5 + 0) | ||||||||
| Cytokines [pg/mL] | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | median | (Min/max) | Median | (Min/max) | Median | (Min/max) |
| IFNg_PWM | 116.7 | (0/7816.5) | 180.6 | (0.8/3898.5) | 116.8 | (0.2/7816.5) | 147.6 | (0/3898.5) | 4.5 | (0.5/983.9) | 178.6 | (2.2/1073.7) | 4.5 | (0.5/983.9) | 178.6 | (2.2/1073.7) |
| IFNg_P + C | 92.8 | (0.3/7215) | 71.2 | (0.3/1280.4) | 113.6 | (0.6/7215) | 56.0 | (0.3/1280.4) | 126.9 | (1.1/1009.9) | 81.6 | (2/1081) | 126.9 | (1.1/1009.9) | 81.6 | (2/1081) |
| IFNg_HSP70 | 1.5 | (0/7282) | 0.3 | (0/781.9) | 1.1 | (0/7282) | 0.3 | (0/781.9) | 1.1 | (0.1/550.5) | 0.3 | (0.2/552.4) | 1.1 | (0.1/550.5) | 0.3 | (0.2/552.4) |
| IFNg_Pg | 0.9 | (0/633.3) | 1.6 | (0/9.2) | 0.7 | (0/633.3) | 2.1 | (0/9.2) | 0.6 | (0/4.8) | 0.4 | (0.2/1) | 0.5 | (0/4.8) | 0.4 | (0.2/1) |
| IFNg_Aa | 2.9 | (0/230.1) | 1.1 | (0/405.2) | 2.9 | (0/230.1) | 1.1 | (0/405.2) | 1.0 | (0/119.1) | 2.1 | (0.2/240) | 1.0 | (0/119.1) | 3.2 | (0.2/240) |
| IFNg_Tf | 2.6 # | (0.1/633.3) # | 0.4 | (0/9.9) | 2.1 * | (0.1/633.3) * | 0.4 | (0/9.9) | 1.6 | (0/89.4) | 0.3 | (0.1/99.5) | 0.4 | (0/89.4) | 0.4 | (0.1/99.5) |
| IFNg_PI | 0.8 | (0/238.7) | 0.2 | (0/1.9) | 0.8 | (0/238.7) | 0.3 | (0/2.2) | 0.7 | (0/61.1) | 0.2 | (0.1/809.9) | 0.3 | (0/61.1) | 0.2 | (0.1/809.9) |
| IFNg_unstim. | 0.7 | (0/21.9) | 0.1 | (0/8.6) | 0.6 | (0/21.9) | 0.1 | (0/8.6) | 0.0 | (0/91) | 0.1 | (0/7) | 0.0 | (0/91) | 0.1 | (0.1/0.4) |
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| IL-10_PWM | 63.5 | (0/3447) | 60.3 | (0/518.6) | 90.3 | (0/3447) | 55.1 | (0/518.6) | 76.0 | (0/1017) | 85.7 | (37.4/326) | 76.0 | (0/1017) | 180.7 | (37.4/326) |
| IL-10_P + C | 37.2 | (0/866.7) | 30.4 | (0/1732.9) | 41.0 | (0/866.7) | 27.6 | (0/1732.9) | 125.9 | (8.8/497.2) | 105.1 | (26.3/537.7) | 125.9 | (8.8/497.2) | 105.1 | (26.3/537.7) |
| IL-10_HSP70 | 5.1 | (0/581.3) | 1.7 | (0/271.7) | 5.6 | (0/581.3) | 3.2 | (0/271.7) | 9.7 | (1.3/250.3) | 10.3 | (3.9/215.4) | 10.4 | (1.3/250.3) | 4.8 | (3.9/215.4) |
| IL-10_Pg | 2.5 | (0/164.5) | 2.3 | (0/81.9) | 1.8 | (0/164.5) | 3.6 | (0/81.9) | 0.5 | (0/45.7) | 0.3 | (0.1/9) | 0.5 | (0/45.7) | 0.3 | (0.1/6.2) |
| IL-10_Aa | 28.8 | (0.8/823.6) | 25.2 | (0.1/589.9) | 36.8 | (0.8/823.6) | 24.0 | (0.1/589.9) | 26.6 | (11.2/303.1) | 58.2 | (4.4/663.1) | 26.6 | (4.4/303.1) | 92.5 | (10.7/663.1) |
| IL-10_Tf | 68.8† | (3.6/1510.4)† | 18.2 | (0.1/374.8) | 106.9† | (9.4/1510.4)† | 20.6 | (0.1/374.8) | 134.2 * | (4.3/320.1) * | 52.9 | (0.6/151.6) | 92.6 | (0.6/320.1) | 63.8 | (0.7/151.6) |
| IL-10_PI | 25.0 # | (0/207.2) # | 2.7 | (0/27.6) | 22.7 * | (0/207.2) * | 3.0 | (0/81.9) | 9.2 | (0/173.6) | 3.7 | (0.4/29.1) | 5.7 | (0/173.6) | 5.5 | (1.1/29.1) |
| IL-10_unstim. | 2.7 | (0/14.7) | 0.4 | (0/7.2) | 2.0 | (0/14.7) | 0.6 | (0/7.2) | 0.4 | (0/11) | 2.1 | (0.7/6.2) | 0.7 | (0/11) | 2.8 | (0.7/6.2) |
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| IL-17_PWM | 8.0 | (0/2200) | 15.0 | (5.1/1248.5) | 8.5 | (0.5/2200) | 13.5 | (0/1248.5) | 47.2 | (0.8/2064.1) | 127.0 | (2.3/947.3) | 47.2 | (0.8/2064.1) | 127.0 | (3/947.3) |
| IL-17_P + C | 34.4 | (1.1/2200) | 62.1 | (2.8/2200) | 50.2 | (1.1/2200) | 42.2 | (1.5/2200) | 239.2 | (2.8/2200) | 140.4 | (0.7/2376.8) | 239.2 | (0.7/2200) | 140.4 | (1.1/2376.8) |
| IL-17_HSP70 | 6.3 | (0/1107.5) | 1.2 | (0/968.6) | 4.0 | (0/1107.5) | 1.4 | (0/968.6) | 7.2 | (0.1/349.5) | 0.3 | (0.1/737.9) | 7.2 | (0.1/349.5) | 0.3 | (0.1/737.9) |
| IL-17_Pg | 1.1 | (0/543.1) | 0.4 | (0/12.9) | 1.0 | (0/543.1) | 1.2 | (0/12.9) | 0.1 | (0/6.9) | 0.3 | (0.1/0.6) | 0.1 | (0/6.9) | 0.3 | (0.1/0.4) |
| IL-17_Aa | 2.6 | (0.8/88.3) | 1.1 | (0.1/45.1) | 2.4 | (0.8/88.3) | 1.9 | (0.1/45.1) | 0.3 | (0/10.2) | 1.2 | (0/72.2) | 0.4 | (0/10.2) | 1.2 | (0/72.2) |
| IL-17_Tf | 1.6 | (0/2064.1) | 1.5 | (0/22.6) | 1.5 | (0/2064.1) | 2.1 | (0/22.6) | 0.7 | (0/11.6) | 0.4 | (0/168.9) | 0.3 | (0/11.6) | 1.4 | (0/168.9) |
| IL-17_PI | 1.5 | (0/62.1) | 0.0 | (0/6.3) | 1.5 | (0/62.1) | 0.1 | (0/6.3) | 0.4 | (0/10.2) | 0.4 | (0/2.2) | 0.3 | (0/10.2) | 1.5 | (0/2.2) |
| IL-17_unstim. | 1.2 | (0/5.1) | 0.5 | (0/6.3) | 1.0 | (0/5.1) | 0.8 | (0/6.3) | 0.2 | (0/2.8) | 0.3 | (0/22.7) | 0.2 | (0/22.7) | 0.3 | (0/2.5) |
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| IL-1 alfa_PWM | 141.3 | (0/2400) | 160.7 | (22/7780) | 152.0 | (0/2400) | 158.7 | (22/7780) | 231.3 | (0/967) | 258.7 | (112/774.1) | 197.1 | (0/967) | 412.2 | (212.2/774.1) |
| IL-1 alfa_P + C | 140.0 | (2.3/4701.5) | 124.6 | (3.6/1643.5) | 140.6 | (4.3/4701.5) | 124.2 | (2.3/1643.5) | 159.9 | (15.4/1153.3) | 247.1 | (121.9/436.8) | 159.9 | (15.4/1153.3) | 261.1 | (242.5/436.8) |
| IL-1 alfa_HSP70 | 32.8 | (0/1139.8) | 13.0 | (0/187.4) | 52.3 | (0/1139.8) | 13.0 | (0/187.4) | 24.1 | (6.4/398.9) | 124.0 * | (43.3/496.4) * | 31.1 | (6.4/398.9) | 124.0 | (43.3/496.4) |
| IL-1 alfa_Pg | 47.5 | (4.3/635.4) | 44.0 | (1.9/339) | 47.5 | (4.4/635.4) | 44.7 | (1.9/339) | 38.4 | (6.8/372.5) | 69.9 | (53.8/263.5) | 44.2 | (6.8/372.5) | 63.8 | (53.8/263.5) |
| IL-1 alfa_Aa | 97.2 | (11.2/724.2) | 99.3 | (2.8/1745.6) | 97.2 | (13.3/724.2) | 110.5 | (2.8/1745.6) | 129.7 | (13/238.7) | 350.3 * | (108.3/821.5) * | 133.5 | (13/238.7) | 405.3 * | (167.8/821.5) * |
| IL-1 alfa_Tf | 106.3 | (0/450.7) | 37.7 | (3.3/188.4) | 106.3 | (0/450.7) | 46.7 | (3.3/194.9) | 121.4 | (18.6/720.9) | 236.9 | (0.2/465.6) | 111.5 | (18.6/720.9) | 355.3 | (0.2/465.6) |
| IL-1 alfa_PI | 134.2 | (15.9/248.2) | 17.0 | (1.9/238.7) | 133.3 | (15.9/248.2) | 34.4 | (1.9/238.7) | 84.4 | (11.9/218.5) | 242.3 | (11.9/346.4) | 84.6 | (11.9/346.4) | 242.3 | (11.9/287) |
| IL-1 alfa_unstim. | 17.4 | (0/185.5) | 6.8 | (0/397.3) | 18.8 * | (0/185.5) * | 9.0 | (0/397.3) | 20.8 | (2.3/120.6) | 140.2 * | (5.9/308.8) * | 22.5 | (2.3/178.1) | 140.2 | (5.9/308.8) |
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| IL-1 beta_PWM | 583.3 | (0/2586) | 267.1 | (0/1597) | 655.9 * | (0/2586) * | 214.2 | (0/1597) | 676.4 | (2.6/1600) | 839.7 | (100.9/1700) | 676.4 | (2.6/1600) | 839.7 | (285.3/1700) |
| IL-1 beta_P + C | 533.3 | (1.3/2650) | 278.3 | (1.6/1600) | 600.2 * | (2.6/2650) * | 266.5 | (1.3/1600) | 695.2 | (47.2/1600) | 610.5 | (168.2/1657) | 571.9 | (47.2/1600) | 733.8 | (324.8/1657) |
| IL-1 beta_HSP70 | 141.5 | (0/1600) | 34.7 | (0/616.1) | 228.9 * | (0/1600) * | 34.7 | (0/616.1) | 93.6 | (44.4/1600) | 335.9 | (183.2/1338.1) | 143.4 | (44.4/1600) | 335.9 | (198.5/1338.1) |
| IL-1 beta_Pg | 33.2 | (0/851.4) | 13.8 | (0/600.4) | 33.2 | (0/851.4) | 16.3 | (0/600.4) | 22.9 | (3.7/106) | 8.2 | (2.4/289.7) | 22.9 | (2.9/289.7) | 8.2 | (2.4/189.5) |
| IL-1 beta_Aa | 533.3 | (0/4216.5) | 482.3 | (0/1600) | 533.3 * | (0/4216.5) * | 405.2 | (0/1600) | 439.5 | (47.7/1271.5) | 483.6 | (167.5/1531.1) | 439.5 | (47.7/1271.5) | 748.0 | (344.3/1531.1) |
| IL-1 beta_Tf | 516.8 * | (0/2952) * | 109.6 | (0/952.7) | 533.3 # | (0/2952) # | 101.8 | (0/952.7) | 521.6 | (47.7/1600) | 448.5 | (0/1700) | 479.4 | (47.7/1600) | 558.8 | (0/1700) |
| IL-1 beta_PI | 251.0 | (0/552.8) | 63.8 | (0/578.6) | 244.7 | (0/552.8) | 68.7 | (0/578.6) | 302.9 | (145.5/985) | 342.0 | (44.4/748.2) | 302.9 | (145.5/985) | 390.8 | (44.4/748.2) |
| IL-1 beta_unstim. | 83.5 * | (0/968.5) * | 21.9 | (0/533.3) | 91.8 * | (0/968.5) * | 16.6 | (0/533.3) | 66.0 | (9.5/621.8) | 195.2 | (21.5/415.6) | 66.0 | (9.5/621.8) | 247.7 | (21.5/415.6) |