(b).
| Locus | Periodontitis patients | Healthy controls | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IL-4-590 | IL-4VNTR | IL-4-590 | IL-4VNTR | |||||||||||||
| Polymorphism | CC | CT + TT | 11 | 12 + 22 | CC | CT + TT | 11 | 12 + 22 | ||||||||
| Number of patients | (30) | (16 + 1) | (28) | (17 + 1) | (8) | (5 + 2) | (10) | (5 + 0) | ||||||||
| Cytokines [pg/mL] | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) | Median | (Min/max) |
| IL-4_PWM | 3.6 | (0/163.6) | 3.9 | (0/17.8) | 3.7 | (0/163.6) | 3.4 | (0/17.8) | 4.3 | (0.6/163.6) | 3.6 | (0.7/9.9) | 4.3 | (0.6/163.6) | 3.6 | (0.7/9.9) |
| IL-4_P + C | 3.7 | (0/40.7) | 4.4 | (0/25.7) | 4.0 | (0/40.7) | 4.0 | (0/25.7) | 0.9 | (0/20.1) | 2.2 | (0.7/19.9) | 0.9 | (0/20.1) | 2.2 | (0.7/11.9) |
| IL-4_HSP70 | 0.7 | (0/6.5) | 0.6 | (0/5.5) | 0.0 | (0/6.5) | 1.2 | (0/5.5) | 1.2 | (0/3.1) | 1.6 | (0.7/2.3) | 1.2 | (0/3.1) | 1.6 | (0.7/2.3) |
| IL-4_Pg | 0.5 | (0/12.9) | 0.1 | (0/7.7) | 0.1 | (0/12.9) | 0.1 | (0/7.7) | 0.7 | (0/96.6) | 0.7 | (0/1.2) | 0.7 | (0/96.6) | 0.7 | (0.6/1.2) |
| IL-4_Aa | 0.4 | (0/11.2) | 0.1 | (0/3.7) | 0.1 | (0/4.7) | 0.1 | (0/11.2) | 1.9 | (0.7/113.8) | 0.7 | (0/4.5) | 1.9 | (0.7/113.8) | 0.7 | (0/4.5) |
| IL-4_Tf | 1.8 | (0/113.8) | 0.8 | (0/3.7) | 0.1 | (0/113.8) | 1.6 | (0/96.6) | 1.2 | (0/5.7) | 1.2 | (0.7/2.6) | 1.2 | (0/5.7) | 1.2 | (0.7/2.6) |
| IL-4_PI | 0.7 | (0/4) | 0.0 | (0/3.7) | 0.3 | (0/4) | 0.6 | (0/3.7) | 0.7 | (0/4.1) | 0.7 | (0/1.9) | 0.7 | (0/4.1) | 0.7 | (0/1.9) |
| IL-4_unstim. | 0.0 | (0/3.7) | 0.0 | (0/3.3) | 0.0 | (0/3.7) | 0.0 | (0/3.3) | 0.9 | (0/2.8) | 0.7 | (0/4.5) | 0.9 | (0/4.5) | 0.7 | (0/2.6) |
|
| ||||||||||||||||
| IL-5_PWM | 5.7 | (0/1882) | 3.7 | (0/98.9) | 8.4 | (0/1882) | 1.4 | (0/98.9) | 7.3 | (0.6/212.4) | 10.6 | (1.8/143) | 7.3 | (0.6/212.4) | 10.6 | (1.8/112.4) |
| IL-5_P + C | 8.7 | (0/230.1) | 9.5 | (0/375.5) | 9.0 | (0/230.1) | 6.9 | (0/375.5) | 20.0 | (2.3/226.2) | 9.2 | (1.2/403.2) | 20.0 | (2.3/403.2) | 9.2 | (1.2/169.3) |
| IL-5_HSP70 | 1.5 | (0/64.8) | 0.3 | (0/61) | 1.0 | (0/64.8) | 0.6 | (0/61) | 3.8 | (0.6/158) | 6.0 | (1.8/16.9) | 4.3 | (0.6/158) | 5.2 | (1.8/16.9) |
| IL-5_Pg | 1.0 | (0/322.7) | 1.2 | (0/8.1) | 0.9 | (0/322.7) | 1.2 | (0/8.1) | 3.0 | (0.6/6) | 3.8 | (3.2/7.7) | 3.5 | (0.6/7.7) | 3.7 | (3.2/6) |
| IL-5_Aa | 1.7 | (0/12.5) | 1.0 | (0/7.9) | 1.5 | (0/12.5) | 1.2 | (0/7.9) | 3.3 | (2.2/5.2) | 5.2 | (0/6) | 3.3 | (2.2/6) | 5.2 | (0/6) |
| IL-5_Tf | 1.9 | (0/212.4) | 0.6 | (0/3.7) | 1.8 | (0/212.4) | 1.2 | (0/3.9) | 2.0 | (1.2/5.2) | 2.9 | (1.2/6) | 2.4 | (1.2/6) | 2.9 | (1.2/6) |
| IL-5_PI | 1.5 | (0/28.6) | 0.6 | (0/3.7) | 1.7 | (0/28.6) | 0.6 | (0/3.7) | 2.4 | (0.6/4.2) | 3.4 | (0.6/6.9) | 2.9 | (0.6/4.7) | 3.4 | (0.6/6.9) |
| IL-5_unstim. | 0.7 | (0/8.1) | 0.7 | (0/7.9) | 0.6 | (0/8.1) | 0.9 | (0/7.9) | 2.5 | (0/6) | 3.4 | (0/6.9) | 2.5 | (0/6) | 3.4 | (1.8/6.9) |
|
| ||||||||||||||||
| IL-6_PWM | 3500.0 | (0/5960) | 3500.0 | (0/4699) | 3500.0 | (5.4/5960) | 3500.0 | (0/4699) | 2143.1 | (0/4824.3) | 3281.8 | (130.5/5200) | 2143.1 | (0/5200) | 3281.8 | (1186.1/4272.5) |
| IL-6_P + C | 3196.5 | (0/6457) | 2280.7 | (44.7/5528.5) | 3500.0 | (9.3/6457) | 2267.5 | (0/5528.5) | 2876.5 | (211.1/4900) | 1593.1 | (123.2/5193.4) | 2876.5 | (211.1/5193.4) | 1593.1 | (123.2/4931.7) |
| IL-6_HSP70 | 773.9 | (3.3/4207) | 141.4 | (2.7/5833.5) | 1143.1 | (3.3/4207) | 51.9 | (2.7/5833.5) | 888.5 | (165.6/3500) | 2040.7 | (167.5/4673.4) | 888.5 | (165.6/4673.4) | 2040.7 | (167.5/3111.3) |
| IL-6_Pg | 19.8 | (0/4191.6) | 10.6 | (0.4/3577.2) | 19.8 | (0/4191.6) | 11.5 | (0.4/3577.2) | 13.8 | (0/791) | 6.5 | (0.3/2438.4) | 13.8 | (0/2438.4) | 6.5 | (0.3/1675.2) |
| IL-6_Aa | 3500.0 | (27.5/6667) | 3500.0 | (71.9/7362.5) | 3500.0 | (839/6667) | 3500.0 | (27.5/7362.5) | 3500.0 | (108/4686) | 2076.7 | (258.5/3600) | 3366.9 | (108/4686) | 2076.7 | (1802.6/3600) |
| IL-6_Tf | 3500.0 | (25.1/6760) | 1035.3 | (1.4/4916) | 3500.0 * | (108/6760) * | 913.9 | (1.4/4916) | 3457.2 | (33.9/4203.3) | 2037.2 | (0.2/3500) | 3449.9 | (33.9/4203.3) | 2037.2 | (0.2/3500) |
| IL-6_PI | 1052.6 * | (336.1/4032) * | 350.9 | (0.7/3521.6) | 1102.1 * | (336.1/4032) * | 585.5 | (0.7/3521.6) | 3011.5 | (6.2/4686) | 324.3 | (87.5/1937.7) | 2387.8 | (6.2/4686) | 324.3 | (87.5/1337) |
| IL-6_unstim. | 214.8 | (2/3200.5) | 20.0 | (1/2051.9) | 227.2* | (2/3200.5)* | 8.0 | (1/2051.9) | 468.4 | (33.9/1796.2) | 558.5 | (14.2/2046.3) | 418.9 | (14.2/1796.2) | 601.9 | (25.2/2046.3) |
|
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| TNF alfa_PWM | 1085.9 | (2.8/3701) | 1002.3 | (0/3880) | 1250.1 | (3.5/3701) | 963.4 | (0/3880) | 1506.6 | (3.5/2800) | 1209.1 | (85.1/2800) | 1494.2 | (3.5/2800) | 1209.1 | (85.1/2800) |
| TNF alfa_P + C | 1071.5 | (0/3871) | 450.2 | (74/2800) | 1127.2 | (7.3/3871) | 442.1 | (0/2800) | 1579.4 | (35.6/2800) | 1531.3 | (22.9/2800) | 1596.6 | (35.6/2800) | 1531.3 | (22.9/2800) |
| TNF alfa_HSP70 | 32.1 | (2.6/3494.6) | 11.6 | (2.3/2800) | 92.6 | (2.6/3494.6) | 10.6 | (2.3/2800) | 63.4 | (9.6/1249.1) | 152.2 | (11.8/1656.7) | 87.6 | (9.6/1656.7) | 152.2 | (11.8/432) |
| TNF alfa_Pg | 20.6 | (0.5/612.2) | 13.6 | (1.9/576.6) | 21.1 | (0.5/612.2) | 13.3 | (1.9/576.6) | 2.1 | (0.3/54.6) | 0.8 | (0.2/146.3) | 2.1 | (0.2/146.3) | 0.8 | (0.4/51.8) |
| TNF alfa_Aa | 407.1 | (10.7/4760) | 462.2 | (3.7/3880) | 510.8 | (10.7/4760) | 441.8 | (3.7/3880) | 933.0 | (104.4/3569.7) | 527.6 | (51.9/4347.3) | 933.0 | (51.9/3569.7) | 527.6 | (235.7/4347.3) |
| TNF alfa_Tf | 650.9 | (4.2/2845.4) | 179.7 | (2.1/2393.9) | 687.5 * | (104.4/2845.4) * | 147.2 | (2.1/2393.9) | 899.8 | (8.5/1813.5) | 387.6 | (0.1/965.3) | 886.2 | (8.5/1813.5) | 387.6 | (0.1/965.3) |
| TNF alfa_PI | 74.0 | (8.3/1448.7) | 39.1 | (1.2/182.6) | 73.4 | (8.3/1448.7) | 55.0 | (1.2/297.7) | 80.5 | (22.2/130.7) | 38.2 | (9.5/223) | 73.4 | (10.4/130.7) | 38.2 | (9.5/223) |
| TNF alfa_unstim. | 14.0 | (1.8/2336.7) | 6.6 | (1.7/91.8) | 16.7 | (1.8/2336.7) | 4.8 | (1.7/91.8) | 7.0 | (2.1/52.1) | 21.1 | (3.5/112.1) | 10.5 | (2.1/52.1) | 18.7 | (3.5/112.1) |
|
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| VEGF_PWM | 13.7 | (3.4/66.9) | 5.5 | (1.5/94.4) | 14.4 | (3.4/66.9) | 5.1 | (1.5/94.4) | 17.1 | (0.2/83.4) | 4.9 | (0.1/33.1) | 11.0 | (0.2/83.4) | 11.9 | (0.1/33.1) |
| VEGF_P + C | 21.7 | (4.7/96.1) | 9.4 | (1.6/52.6) | 27.2 | (4.7/96.1) | 9.3 | (1.6/52.6) | 12.3 | (0/20.6) | 5.2 | (0.2/17.2) | 9.6 | (0/20.6) | 5.2 | (0.2/17.2) |
| VEGF_HSP70 | 32.8 | (5.8/101.1) | 10.6 | (1.1/45.3) | 35.5 | (5.8/101.1) | 16.0 | (1.1/45.3) | 54.0 | (3/121.7) | 14.4 | (1.5/91.9) | 42.7 | (3/121.7) | 15.7 | (1.5/91.9) |
| VEGF_Pg | 17.9 | (0.5/48.1) | 9.4 | (0.8/63.6) | 17.4 | (0.5/48.1) | 15.3 | (0.8/63.6) | 2.9 | (0.3/33.7) | 5.5 | (0.1/30) | 5.1 | (0.3/33.7) | 2.1 | (0.1/30) |
| VEGF_Aa | 27.4 | (8.8/119.1) | 27.7 | (3.5/57.8) | 27.0 | (8.8/119.1) | 29.5 | (3.5/57.8) | 47.8 | (4.1/73.7) | 27.8 | (7.6/36.8) | 23.9 | (4.1/73.7) | 30.4 | (7.6/36.8) |
| VEGF_Tf | 18.4 | (4.1/89.4) | 17.0 | (4/90.1) | 17.2 | (4.1/89.4) | 19.0 | (4/90.1) | 18.9 | (3.6/126.8) | 11.1 | (0.1/27.3) | 10.6 | (3/126.8) | 13.9 | (0.1/27.3) |
| VEGF_PI | 30.2 | (7.1/94.4) | 15.2 | (4.6/72.8) | 29.1 | (7.1/94.4) | 22.6 | (4.6/72.8) | 30.2 | (20/97.8) | 15.2 | (0.1/49.9) | 29.4 | (10/97.8) | 15.2 | (0.1/49.9) |
| VEGF_unstim. | 24.9 | (0.9/91) | 22.8 | (4.5/135.5) | 29.6 | (0.9/91) | 21.9 | (4.5/135.5) | 39.8 | (15.3/79.4) | 10.2 | (6.4/42.4) | 35.6 | (9.5/79.4) | 10.2 | (6.4/42.4) |
* , #, and † denote significantly higher levels of cytokines for P < 0.05, P < 0.01, and P < 0.001 (resp.), how they were found by Kruskal-Wallis tests in application to couple locus in table header (the minor homozygous locus was connected to a heterogeneous locus for the formal increase the statistical significance of results).