Table 1.
Initial screening of the inhibitory effects of ophiopogonin D (OD), ophiopogonin D′ (OD′), methylophiopogonanone A (MA), methylophiopogonanone A (MB), and ruscorectal (RU). 100 μM of compounds was selected. The values shown in this table are residual activity which can be calculated using the following equation: % residual activity = the activity at 100 μM of compounds/the activity at 0 μM of compounds.
| OD | OD′ | MA | MB | RU | |
|---|---|---|---|---|---|
| UGT1A1 | 77.1 ± 3.8 | 81.0 ± 12.6 | 31.3 ± 1.3 | 27.9 ± 11.1 | 41.5 ± 36.9 |
| UGT1A3 | 40.7 ± 0.3 | 36.5 ± 2.5 | 35.5 ± 6.1 | 84.2 ± 22.8 | 37.2 ± 18.2 |
| UGT1A4 | 24.6 ± 1.6 | 21.0 ± 1.6 | 70.4 ± 3.8 | 81.1 ± 8.0 | 0.0 ± 0.0 |
| UGT1A6 | 12.8 ± 3.1 | 12.1 ± 1.9 | 67.0 ± 1.7 | 65.3 ± 2.0 | 65.9 ± 11.6 |
| UGT1A7 | 143.5 ± 8.7 | 122.2 ± 1.3 | 57.5 ± 10.8 | 83.4 ± 5.9 | 52.4 ± 7.4 |
| UGT1A8 | 17.3 ± 0.9 | 18.9 ± 6.6 | 20.5 ± 2.1 | 85.0 ± 4.5 | 72.0 ± 3.0 |
| UGT1A9 | 135.3 ± 1.5 | 133.8 ± 2.1 | 38.7 ± 0.0 | 68.2 ± 1.5 | 110.7 ± 1.1 |
| UGT1A10 | 26.5 ± 1.5 | 19.8 ± 3.2 | 70.0 ± 9.3 | 96.9 ± 7.3 | 82.0 ± 5.2 |
| UGT2B4 | 101.7 ± 2.6 | 118.0 ± 21.8 | 71.1 ± 1.5 | 73.2 ± 1.2 | 88.4 ± 1.6 |
| UGT2B7 | 85.4 ± 7.3 | 77.4 ± 7.1 | 23.3 ± 16.7 | 82.8 ± 14.5 | 124.8 ± 59.0 |
| UGT2B15 | 36.9 ± 0.0 | 35.5 ± 1.7 | 23.8 ± 2.8 | 56.1 ± 9.8 | 55.5 ± 19.6 |