Table 2.
Signaling nodes measured
| Signaling node | Measured in prior study |
|---|---|
| IFN-α → p-Stat1 |
Yes |
| IFN-α → p-Stat3 |
Yes |
| IFN-α → p-Stat5 |
Yes |
| IFN-α → p-Stat6 |
Yes |
| IL-2 → p-Stat5 |
Yes |
| IL-2 → p-Stat6 |
Yes |
| IL-4 → p-Stat5 |
Yes |
| IL-4 → p-Stat6 |
Yes |
| IL-6 → p-Stat1 |
Yes |
| IL-6 → p-Stat3 |
Yes |
| IL-27 → p-Stat1 |
Yes |
| IL-27 → p-Stat3 |
Yes |
| IL-27 → p-Stat5 |
Yes |
| IL-27 → p-Stat6 |
Yes |
| R848 → p-Erk |
Yes |
| R848 → p-NF-κB |
Yes |
| PMA → p-S6 |
Yes |
| PMA → p-Erk |
Yes |
| Anti-IgD → p-S6 |
No |
| Anti-IgD → p-Erk |
No |
| Anti-IgM → p-ZAP70/p-Syk |
No |
| Anti-IgM → p-Erk |
No |
| Anti-CD3 → p-ZAP70/p-Syk |
No |
| Anti-CD3 → p-Erk | No |
The following 24 signaling nodes (modulator and intracellular readout combinations) were measured in the current study. The majority of these signaling nodes (i.e. the 18 signaling nodes indicated above) were also measured in the previously published study cohort [7]. All signaling nodes were measured in 12 cell populations defined by their surface phenotypes including 7 distinct immune cell subpopulations (monocytes, B cells, CD3-CD20- lymphocytes (NK cell-enriched subpopulation), CD45RA+ Th cells, CD45RA- Th cells, CD45RA+ cytotoxic T cells, and CD45RA- cytotoxic T cells).