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. 2014 Oct 30;2014:283126. doi: 10.1155/2014/283126

Figure 2.

Figure 2

In vitro lipopolysaccharide (LPS) and lipoteichoic acid (LTA) stimulation of neonatal and adult whole blood (WB) in the presence of specific inhibitors. Human WB was preincubated with 20 μM of the p38 MAPK inhibitor SB202190, 20 μM of the ERK1/2 inhibitor U0126, 20 μM of the JNK inhibitor SP600125, or 20 μM of the NF-κB inhibitor BAY11-7082, followed by LPS (100 ng/mL) ((a) and (c)) or LTA (100 μg/mL) ((b) and (d)) for an additional 4 h. Supernatants were then collected, and IL-6 ((a) and (b)) and TNF-α ((c) and (d)) were analyzed using ELISA. Data are presented as fold difference as compared to untreated controls. Values are expressed as mean ± standard error of mean (SEM). * P < 0.05, control versus inhibitor. # P < 0.05, neonatal versus adult.