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. 2014 Oct 30;2014:658589. doi: 10.1155/2014/658589

Figure 2.

Figure 2

LA concentration-dependently modulated mesangial cell-cycle progression stimulated by high glucose. MCs were preincubated in medium with high glucose for 1 h and then stimulated with various concentrations of LA for 24 h, respectively. LA with final concentrations of 0.1 and 0.25 mM promoted cell-cycle progression induced by high glucose, with a maximum promotion at 0.25 mM, while cell cycle was inhibited as the dose of LA increased above 0.5 mM, with a maximum inhibition at 1 mM. Cells were analyzed by flow cytometry after PI staining and the relative percentage of cells in different cell-cycle phases is reported, while the percentage of apoptotic events was ignored. Data in the bar graphs represent the mean ± SD (n = 3). P < 0.05 was considered statistically significant. Note: * P < 0.05 versus NG; # P < 0.05 versus HG. Abbreviations: NG, normal glucose (5 mM); Man (5 mM glucose + 25 mM mannitol); HG, high glucose (30 mM).