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. Author manuscript; available in PMC: 2015 May 11.
Published in final edited form as: Nat Commun. 2014 Nov 11;5:5401. doi: 10.1038/ncomms6401

Figure 1. Nitrate uptake and reduction in plants with altered NO signalling.

Figure 1

(a) Expression of the nitrate transporter marker genes NRT1.1 and NRT2.1 in roots of WT, nox1 and par2-1 plants was determined by qRT-PCR and normalized to expression of ACT2. Error bars represent SD (n = 3). (b) Effect of GSNO on nitrate-induced expression of NRT genes in roots. WT seedlings grown in half-strength MS medium (9.4 mM KNO3 and 10.3 mM NH4NO3) were incubated for 3 hours in water with 1 mM nitrate (KNO3), in the absence or presence of GSNO or DEA/NO. NRT expression was determined by qRT-PCR and normalized to expression of ACT2. Error bars represent SD (n = 3). (c) NR activity and (d) nitrate (NO3−) content determined in leaf extracts of WT plants and genotypes with enhanced (nox1 and par2-1) or impaired (nia1nia2 and 35S::FLAG-GSNOR1) NO homeostasis, after six hours of light. Data points represent means ± SD of three independent experiments. Asterisks indicate statistical differences from the WT (Student’s t test, P < 0.05).