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. Author manuscript; available in PMC: 2015 May 11.
Published in final edited form as: Nat Commun. 2014 Nov 11;5:5401. doi: 10.1038/ncomms6401

Figure 3. Nitrate-derived NO suppresses activity of GSNOR1.

Figure 3

(a) GSNO Reductase (GSNOR) activity, (b) Nitrate Reductase (NR) activity, (c) NO emission and (d) GSNOR1 gene expression measured in leaf extracts of WT and nia1nia2 plants grown under low or high nitrate availability. Plants were grown in perlite:vermiculite (1:1) under a 12/12 hours light/dark period and irrigated three times a week with MS nutrient solution containing 2.5 mM or 25 mM nitrate (half KNO3 and half NH4NO3). All measurements were taken six hours post light exposure. Relative expression of GSNOR1 in (d) was determined by qRT-PCR and normalized to expression of ACT2. (e) GSNOR activity in genotypes with impaired and enhanced NO signalling. Plants were grown in soil with a photoperiod of 16/8 hours light/dark and irrigated with water as needed. Data points represent means ± SD of three independent experiments. Asterisks indicate significant differences from the WT (Student’s t test, P < 0.05).