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Effects of HDAC inhibitors on the expression of p21Cip1, p27Kip1, and p53 protein. After treatment of larvae with 0.1 μM TSA or 100 μM VPA for 48 h, protein extracts were prepared and subjected to western blot assay using antibodies against p21Cip1, p27Kip1, and p53. β-Actin was included as the control. Mean ± s.e.m. for three experimental replicates. *p < 0.05.
