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IQGAP1 Expression Correlates with VSMC Synthetic Phenotype. The cultured HUVSMCs were incubated with 10 ng/mL PDGF-BB for the indicated times (0, 4 and 8 h). Then, the mRNA levels of IQGAP1 were examined (A). Furthermore, the mRNA levels of SMC-specific markers (SMA, SM and CNN) were analyzed by RT-PCR (B). Antibodies against SMA, SM and CNN were used to detect their protein levels (C).
