Figure 3.

MiR-19a induces an epithelial-to-mesenchymal transition (EMT) phenotype. A. MiR-19a induces an EMT phenotype in SGC7901 and NUGC-3 cells. B. Epithelial and mesenchymal markers ZEB1, ZEB2, Slug, vimentin, and E-cadherin were measured by qRT-PCR from miR-19a mimic or negative control (N.C)-transfected SGC7901 and NUGC-3 cells. The results are shown as fold change compared with negative control. Data are the means ± SD of three independent experiments. *P < 0.05. C. The E-cadherin, vimentin protein levels were measured using western blot analysis. β-actin protein levels served as an internal (loading) control.