Table 1.
Antibodies characterization for immunostaining
| Antibody | Vendor | Clone | Dilution | Antigen Retrieval Solution |
|---|---|---|---|---|
| ER | Roche | SP1 | Using antibody | Tris-based buffer (pH: 8.0) |
| PR | Roche | 1E2 | Using antibody | Tris-based buffer (pH: 8.0) |
| HER-2/neu | Roche | 4B5 | Using antibody | Tris-based buffer (pH: 8.0) |
| Ki-67 | Roche | 30-9 | Using antibody | Tris-based buffer (pH: 8.0) |
| CK5/6 | DAKO | D5/16B4 | 1:200 | Tris-based buffer (pH: 8.0) |
| EGFR | Changdao, China | 111.6 | 1:100 | Tris-based buffer (pH: 8.0) |
| GCDFP15 | DAKO | 23A3 | 1:50 | Citrate buffer (pH: 6.0) |
| MGB | DAKO | 304-1A5 | 1:50 | Citrate buffer (pH: 6.0) |
| AR | DAKO | AR441 | 1:50 | EDTA (pH: 9.0) |
| FOXP1 | Epitomics | EPR4113 | 1:300 | EDTA (pH: 9.0) |