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. 2014 Sep 15;7(10):7249–7261.

Figure 2.

Figure 2

MiR-378 overexpression improves cell viability and mineralization under high glucose. A. Relative qRT-PCR expression levels of miR-378 48 h after transfection in MC3T3-E1, MC3T3-E1/miR-con and MC3T3-E1/miR-378 cells under high glucose (HG, 25.5 mM). B. Mineralization of osteoblasts cultured in HG for 21 d depicted by Alizarin Red S staining. C. Alizarin Red S quantification by absorbance (at 550 nm) of osteoblasts cultured in HG for 21 d. D. Viability of MC3T3-E1, MC3T3-E1/miR-con and MC3T3-E1/miR-378 cells cultured in HG for 24-96 h represented by production of formazan in an MTT assay. E. Representative flow cytometric analysis of apoptosis in MC3T3-E1 cells 48 h after transfection. The apoptosis rates were the values of Q2 quadrant plus Q4 quadrant. *P < 0.05, **P < 0.01.