Table 1.
Antibdy | Type | pre-treated | dilution | location | details |
---|---|---|---|---|---|
PLAP | mouse anti-human | heated | WS | cytoplasm | DaKo (GM719102) |
OCT3/4 | goat anti-IgG | heated | 1:50 | nucleus | Santa Cruz (c-20, sc-8629) |
c-kit | rabbit anti-human | heated | WS | membrane | DaKo (A450202) |
β-HCG | rabbit anti-human | heated | WS | cytoplasm | DaKo (GA023102) |
AFP | rabbit anti-human | None | WS | cytoplasm | DaKo (GA000804) |
CK AE1/3 | mouse anti-human | typsin digestion | 1:50 | cytoplasm | DaKo (GM082110) |
CD30 | mouse anti-human | heated | WS | membrane | DaKo (GM075102) |
WS: working solution; goat anti-mouse, rabbit anti-goat, goat anti-rabbit.