Table 1.
Antibodies used in the present study
| Antibody to | Clone | Dilution | Antigen Retrieval | Source |
|---|---|---|---|---|
| G-CSF | 4-12-2 | 1:100 | HIER | Immuno-Biological Laboratories |
| G-CSFR | Polyclonal | 1:200 | HIER | Bioss |
| AE1/AE3 | Cocktail, AE1 and AE3 | 1:100 | HIER | Novocastra |
| HNF4α | K9218 | 1:100 | HIER | Perseus |
| CK7 | OV-TL 12/30 | 1:50 | HIER | Dako |
| CK20 | Ks 20.8 | 1:50 | HIER | Dako |
| CDX2 | AMT28 | 1:100 | HIER | Novocastra |
| Vimentin | V9 | Prediluted | HIER | Ventana |
| Nestin | N1602 | 1:20 | HIER | Immuno-Biological Laboratories |
| E-cadherin | 36 | Prediluted | HIER | Ventana |
| CD44 | DF1485 | 1:50 | HIER | Novocastra |
| CD133 | AC133 | 1:50 | HIER | Miltenyi |
| Ki-67 | MIB-1 | 1:100 | HIER | Dako |
| p53 | DO-7 | Prediluted | HIER | Ventana |
| EGFR | EGFR PharmDx™ Kit | Dako |
G-CSF: granulocyte colony-stimulating factor; G-CSFR: granulocyte colony-stimulating factor receptor; CK: cytokeratin; EGFR: epidermal growth factor receptor; HIER: heat-induced epitope retrieval; Immuno-Biological Laboratories, Gunma, Japan; Bioss, Massachusetts, USA; Novocastra Laboratories, Newcastle upon Tyne, UK; Perseus Proteomics, Tokyo, Japan; Dako, Glostrup, Denmark; Ventana Medical Systems, Arizona, USA; Miltenyi, California, USA.