Table 3.
Summary of the 10 most abundant oligotypes from each of three oligotyping analysis groupings.
| MEGABLAST results | Percentage of each isolation source category for MEGABLAST hits | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Oligotype ID | Number of 100% hits to nr database | Top species level hits (number of hits to that species) | 1 | 2 | 3 | 4 | 5 | No data | Habitats with Similarity Percentage (SIMPER) results > 10% |
| Oligotype 1 | 22 | V. alfacsensis (6) V. sinaloensis (2) | 45.5 | 9.1 | 40.9 | 0.0 | 0.0 | 4.5 | Sponge/MarineFish/MarineWater |
| Oligotype 2 | 171 | V. metschnikovii (3) V. neptunius (11) | 42.9 | 15.3 | 12.4 | 11.2 | 0.0 | 18.2 | FreshwaterFish/FreshWater |
| Oligotype 3 | 780 | V. scophthalmi (4) V. ichthyoenteri (31) V. anguillarum (37) | 55.7 | 11.4 | 6.9 | 0.1 | 0.0 | 25.9 | MarineFish/MarineWater/Seawater/Saltmarsh/Plastic |
| Oligotype 4 | 39 | V. cholerae (9) V. vulnificus (17) V. mimicus (5) | 2.6 | 0.0 | 0.0 | 0.0 | 30.8 | 66.7 | FreshwaterFish/FreshWater/Sponge/MarineFish |
| Oligotype 5 | 1000* | V. splendidus (52) V. mediterranei (35) V. gigantis (39) | 45.3 | 21.4 | 8.1 | 0.1 | 0.0 | 25.1 | Sand-PAH/Seawater/Saltmarsh/Plastic |
| Oligotype 6 | 23 | V. ichthyoenteri (1) V. ordalii (1) | 70.8 | 8.3 | 0.0 | 0.0 | 0.0 | 20.8 | Sponge/MarineFish/MarineWater |
| Oligotype 7 | 46 | V. ponticus (12) V. nigripulchritudo (9) | 30.4 | 8.7 | 4.3 | 0.0 | 0.0 | 56.5 | Seawater |
| Oligotype 8 | 221 | V. cholerae (184) | 1.4 | 1.4 | 0.5 | 3.6 | 11.8 | 81.4 | FreshwaterFish/FreshWater |
| Oligotype 9 | 1 | V. vulnificus (1) | 0.0 | 0.0 | 0.0 | 0.0 | 100 | 0.0 | |
| Oligotype 10 | 18 | V. alginolyticus (1) | 11.1 | 0.0 | 0.0 | 89.0 | 0.0 | 0.0 | Sand-PAH |
| Oligotype 11 | 1 | None | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 100 | |
| Oligotype 12 | 113 | V. coralliilyticus (2) | 27.4 | 39.8 | 0.0 | 0.0 | 0.0 | 32.7 | Seawater/Plastic |
| Oligotype 13 | 66 | V. azureus (13) V. harveyi (2) | 54.5 | 6.1 | 0.0 | 11.6 | 5.5 | 22.7 | Plastic |
| Oligotype 14 | 0 | V. azureus (5) V. owensii (2) | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 100.0 | |
| Oligotype 15 | 245 | V. vulnificus (4) V. shilonii (3) | 94.5 | 2.4 | 0.4 | 0.0 | 0.0 | 3.4 | |
| Oligotype 16 | 140 | V. kanaloae (3) V. splendidus (2) | 42.1 | 14.3 | 11.4 | 2.1 | 0.0 | 30.0 | |
| Oligotype 17 | 6 | V. splendidus (3) | 84.0 | 0.0 | 0.0 | 0.0 | 0.0 | 16.0 | |
Overlap in the most abundant sequences between groupings reduced the total number to 17. Oligotype names were assigned arbitrarily, but are consistent across all groupings. The species assignments given by reports from 100% MEGABLAST hits, and the number of hits to each species, is shown. The proportion of MEGABLAST hits isolated from each of the five habitat categories are also shown. Categories are; 1. Marine Host, 2. Seawater, 3. Other Marine, 4. Terrestrial or Human, and 5. Freshwater (see Materials and Methods).
(*)
Indicates maximum requested hits.