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. 2014 Nov 13;5:592. doi: 10.3389/fmicb.2014.00592

FIGURE 2.

FIGURE 2

The calcofluor (CF) phenotype of the Sinorhizobium meliloti bluB mutant is influenced by DMB availability and DNA stress. (A) CF fluorescence of S. meliloti bluB on an LB CF plate. Filter disks containing DMB at the indicated concentrations were applied to the plate. (B) CF fluorescence phenotypes of S. meliloti strains (1) bluB mutant expressing Escherichia coli nrdAB on plasmid pMS03 ( Taga and Walker, 2010), (2) Wild type (WT) S. meliloti with empty vector pMS03, (3) bluB mutant with empty vector pMS03, and (4) bluB mutant expressing E. coli metE on plasmid pMS03 ( Taga and Walker, 2010). (C) Strain SmNrdAB+ on an LB CF plate with a filter disk containing hydroxyurea, photographed under white light. (D) Same as (C) but photographed under UV light. (E) Fluorescence of WT S. meliloti on an LB CF plate with a filter disk containing nalidixic acid. Filter disks were applied to the plates at the time of inoculation. (F) Schematic description of the physiological link between the bluB mutation and the CF fluorescence phenotype. The dashed line indicates that the mechanism is unknown.