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. 2014 Nov 13;5:613. doi: 10.3389/fmicb.2014.00613

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Copyright © 2014 Morrill, Brazelton, Kohl, Rietze, Miles, Kavanagh, Schrenk, Ziegler and Lang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The kinetic isotope effect (KIE) of microbial CO utilization using a Rayleigh Distillation Equation (RDE) (Mariotti et al., 1981). The fractionation factor (α) is equal to 1 plus the slope of a linear regression of ln(R/Ro) vs. ln(C/Co) determined from experimental data, where R_o is the initial isotopic ratio (¹³C/¹²C) of the CO and R is the isotopic ratio of the CO at time t, and C_o is the initial concentration of CO and C is the concentration of CO at time t (Scott et al., 2004). Fractionation factors are often reported as enrichment values [ε = 1000 × (α − 1)].