Table 2.

Substrate specificity of A. japonicus LAB01 lipase for various acyl esters of p-nitrophenyl (pNP).

Substrate	Residual activity (%)
C2 (pNPA)	14.18 ± 1.61
C4 (pNPB)	23.42 ± 1.20
C8 (pNPC)	45.21 ± 1.59
C10 (pNPD)	48.10 ± 3.73
C12 (pNPL)	70.42 ± 6.37
C14 (pNPM)	107.09 ± 0.41
C16 (pNPP)	100 ± 0.43

Activities are expressed as per cent of activity against pNPP. The assays were carried out in pH 8.0 at 37°C, preparing different formulations of solution A as described above. Assays were performed in triplicate and mean values are reported.