Table 5.
Effect of organic solvents on the activity/stability of the lipase from A. japonicus LAB01.
| Solvents | Residual activity (%) |
|---|---|
| Hexane | 137.1 ± 4.71 |
| Toluene | 100.9 ± 4.70 |
| Acetonitrile | 0 |
| Acetone | 0 |
| Methanol (25% v/v) | 98.48 ± 0.97 |
| Methanol (50% v/v) | 108.09 ± 0.34 |
| Methanol (75% v/v) | 13.10 ± 0.87 |
| Ethanol (25% v/v) | 90.71 ± 3.89 |
| Ethanol (50% v/v) | 12.65 ± 1.65 |
| Ethanol (75% v/v) | 4.03 ± 0.03 |
| Isopropanol (25% v/v) | 70.65 ± 0.25 |
| Isopropanol (50% v/v) | 2.05 ± 0.19 |
| Isopropanol (75% v/v) | 1.64 ± 0.06 |
Lipase samples were incubated with organic solvents at 30°C for 90 min. The remaining lipase activity relative to the non-solvent containing control was determined on pNPP substrate at pH 8.0 and 37°C. Values are the average of assays carried out in triplicate.