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. 2014 Nov 7;7:97–104. doi: 10.2147/NSA.S70782

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© 2014 Molling et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Study strategy to determine efficacy of different antimicrobial coatings.

Notes: Antimicrobial activity was determined by the golden standard, the ISO 22196:2011 protocol, which comprises three major phases. In the first phase we challenged the antimicrobial coatings (AMC) and control surfaces with a suspension of Escherichia coli. Bacteria were recovered after 24 hours of incubation at 35°C. After 2 days of additional incubation using the pour plate method, the number of CFUs in the obtained suspensions was determined. The killing efficiency of the applied coatings was calculated as described in “Materials and methods”.

Abbreviations: AMC, antimicrobial coating; CFU, colony-forming units; G, glass; ISO, International Organization for Standardization; SS, stainless steel.