Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Oct;10(4):802–809. doi: 10.1128/iai.10.4.802-809.1974

Cellular Immune Activity of a Galactomannan-Protein Complex from Mycelia of Histoplasma capsulatum

E Reiss a,1, W O Mitchell a, S H Stone a, H F Hasenclever a
PMCID: PMC423025  PMID: 4214782

Abstract

A galactomannan-protein complex was extracted from defatted mycelium of Histoplasma capsulatum with 0.25 N alkali at 25 C. It accounted for 7.5% of the cell dry weight in nondialyzable form and had a galactose-mannose ratio of 2:5. The complex containing 68% protein was separated into polysaccharide and glycoprotein components by ion-exchange chromatography. The galactomannan formed a single precipitate in immunodiffusion, sharing a common antigen with the glycoprotein which contained two precipitating antigens. The glycoprotein elicited delayed-type hypersensitivity in guinea pigs as measured by skin-test and macrophage migration inhibitory factor assay, with 5 μg resulting in 86% specific inhibition. The galactomannan was skin-test negative, but 10 μg resulted in 80% specific inhibition of migration.

Full text

PDF
809

Images in this article

805Image
on p.805
807Image
on p.807
808Image
on p.808

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARKER S. A., CRUICKSHANK C. N., MORRIS J. H., WOOD S. R. The isolation of trichophytin glycopeptide and its structure in relation to the immediate and delayed reactions. Immunology. 1962 Nov;5:627–632. [PMC free article] [PubMed] [Google Scholar]
  2. Domer J. E., Hamilton J. G., Harkin J. C. Comparative study of the cell walls of the yeastlike and mycelial phases of Histoplasma capsulatum. J Bacteriol. 1967 Aug;94(2):466–474. doi: 10.1128/jb.94.2.466-474.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FELTON L. D., PRESCOTT B., KAUFFMANN G., OTTINGER B. Pneumococcal antigenic polysaccharide substances from human tissues. J Immunol. 1956 Jan;76(1):69–77. [PubMed] [Google Scholar]
  4. Garcia J. P., Howard D. H. Characterization of antigens from the yeast phase of Histoplasma capsulatum. Infect Immun. 1971 Aug;4(2):116–125. doi: 10.1128/iai.4.2.116-125.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goodman N. L., Spouse R. F., Larsh H. W. Histoplasmin potency as affected by culture age. Sabouraudia. 1968 Oct;6(4):273–284. doi: 10.1080/00362176885190551. [DOI] [PubMed] [Google Scholar]
  6. HEINER D. C. Diagnosis of histoplasmosis using precipitin reactions in agargel. Pediatrics. 1958 Oct;22(4 Pt 1):616–627. [PubMed] [Google Scholar]
  7. Kidby D. K., Davies R. Invertase and disulphide bridges in the yeast wall. J Gen Microbiol. 1970 Jun;61(3):327–333. doi: 10.1099/00221287-61-3-327. [DOI] [PubMed] [Google Scholar]
  8. Kobayashi G. S., Guiliacci P. L. Cell wall studies of Histoplasma capsulatum. Sabouraudia. 1967 Feb;5(3):180–188. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lloyd K. O. Isolation, characterization, and partial structure of peptido galactomannans from the yeast form of Cladosporium werneckii. Biochemistry. 1970 Aug 18;9(17):3446–3453. doi: 10.1021/bi00819a025. [DOI] [PubMed] [Google Scholar]
  11. Reiss E., Stone S. H., Hasenclever H. F. Serological and cellular immune activity of peptidoglucomannan fractions of Candida albicans cell walls. Infect Immun. 1974 May;9(5):881–890. doi: 10.1128/iai.9.5.881-890.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970 Dec 29;222(3):565–582. doi: 10.1016/0304-4165(70)90182-0. [DOI] [PubMed] [Google Scholar]
  13. SALVIN S. B., SMITH R. F. Antigens from the yeast phase of Histoplasma capsulatum. III. Isolation, properties, and activity of a protein-carbohydrate complex. J Infect Dis. 1959 Jul-Aug;105(1):45–53. doi: 10.1093/infdis/105.1.45. [DOI] [PubMed] [Google Scholar]
  14. Sprouse R. F., Goodman N. L., Larsh H. W. Fractionation, isolation and chemical characterization of skin test active components of histoplasmin. Sabouraudia. 1969 Feb;7(1):1–11. doi: 10.1080/00362177085190021. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES