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. Author manuscript; available in PMC: 2014 Nov 13.
Published in final edited form as: Birth Defects Res A Clin Mol Teratol. 2012 Nov 27;97(1):8–27. doi: 10.1002/bdra.23099

Figure 4. Ethanol exposure from 10–18 hpf induces more severe GAD1 expression phenotypes than ethanol exposure from 6–10 hpf.

Figure 4

(A–Jii) Analysis of GAD1 mRNA expression in 1 dpf embryos (lateral view, A, C, E, G and I) and 3 dpf embryos (lateral view and dorsal view respectively, Bi-Bii, Di-Dii, Fi-Fii, Hi-Hii and Ji-Jii). (A, B) WT; (C, D) 6–10 hpf 1.5% ethanol; (E, F) 6–10 hpf 2% ethanol; (G, H) 10–18 hpf 1.5% ethanol; (I, J) 10–18 hpf 2% ethanol. GAD1 expression in (C–Fii, Hi-Hii) is similar to that in WT (A–Bii). Decreased GAD1 expression is observed in (G and I–Jii). The ratio of the embryos from different ethanol exposure times and concentrations that show the above abnormal phenotypes is shown in Table 2.