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. 2014 Oct 24;53(44):6857–6859. doi: 10.1021/bi5012354

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Copyright © 2014 American Chemical Society

This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

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(a) Circular dichroism spectra of wild-type FtsH (wt-FtsH, top) and FtsH-S6 ATPase (bottom). (b) Co-purification of wild-type gankyrin-His_6x and FtsH-S6 ATPase mutants: wt-S6 FtsH-S6 ATPase (lane 1), R342A (lane 2), R338A/R342A (lane 3), R338A/R339A/R342A (lane 4), E356A/E357A (lane 5), D359A/D362A (lane 6), and K397E (lane 7). (c) Co-purification of wild-type S6 ATPase and gankyrin-His_6x mutants: wt-gankyrin (lane 1), R41A (lane 2), K116A (lane 3), R41A/K116A (lane 4), D39A/D71A (lane 5), and E182A (lane 6).