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. 2014 Nov 3;14:796. doi: 10.1186/1471-2407-14-796

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© Schwarzenbach et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Histone signature at the MAGE-A1 promoter as examined by chromatin immunoprecipitation. DNA was derived from unstimulated (basal) MCF-7 cells, 5-aza-CdR- and/or TSA-stimulated MCF-7 cells and MCF-7 cells transfected with the expression plasmid encoding for BORIS. DNA-bound histones were immunoprecipitated by antibodies specific for methylated and acetylated histones, and amplified in a real-time PCR by a primer pair specific for the MAGE-A1 promoter. The background of the non-specific IgG immunoprecipitation was subtracted from the calculated ratio between the data derived from the histone-specific immunoprecipitation and input DNA. H3K9, Lysine 9 of histone H3; H4K8, Lysine 8 of histone H4; H3K4, Lysine 4 of histone H3; H4K20, Lysine 20 of histone H4; ac, acetylated; me, monomethylated; me2, dimethylated; me3, trimethylated. The significant p-values are shown.