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. 2014 Nov 3;14:796. doi: 10.1186/1471-2407-14-796

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© Schwarzenbach et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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BORIS and Sp1 interact with the TATA binding protein (hTBP). The methionine labeled “prey” fusion proteins Sp1, hTBP and the carboxyl terminus of hTBP (hTBP-C) were incubated with various resin-bound “bait” proteins as indicated below the polyacrylamide gels. The protein complexes were collected by precipitation and separated by gel electrophoresis. The weak band in the TNT-mix (negative control) is unspecific and serves as a background level (A). Schematic view of the detected protein-protein interactions. N, amino terminus; C, carboxyl terminus (B).