Table 3.
Culture surface coating requirements and costing for the generation of 1 billion (x10 9 ) hPSCs
| Surface | Manufacturer | Coating density source | Cost per cm2($USD)1 | Cost per 1 trillion cells (5000 cm2) ($USD) |
|---|---|---|---|---|
| MatrigelTM | Becton Dickinson | Becton Dickinson handbook | $0.080 | $400 |
| Cat No: 354277 | ||||
| GeltrexTM | Invitrogen | Life TechnologiesTM handbook | $0.062 | $310 |
| Cat No: A1413302 | ||||
| Recombinant human laminin-511 (whole protein) | BioLamina | [112]2 | $10.7 | $53331 |
| Cat No: LN511 | ||||
| Recombinant human vitronectin (truncated protein) | Gibco® | [73] | $0.0413 | $205 |
| Cat No: A14701SA | ||||
| Recombinant human fibronectin | Abcam | [113] | $0.664 | $3320 |
| Cat No: AB92798 | ||||
| Corning SynthemaxTM II-SC | Corning Inc. | Corning handbook | $0.205 | $1026 |
| Cat No: 3535XX1 | ||||
| StemAdhereTM(E-cadherin fusion protein) | Primorigen Biosciences ® Cat No: S2112 | Primorigen handbook | $0.081 | $406 |
Calculations were based on a typical cell density of 2x105 cells/cm2. These calculations do not take into account requirements for media or plasticware or the implementation of cardiomyocyte differentiation protocols. It should also be noted that such protocols are not 100% efficient, so it is likely that additional cells would be required.
1Prices were obtained from the websites of Australian suppliers of the manufacturers listed and converted from $AUD to $USD on the ninth of April 2013 ($1AUD =1.04$USD).
2Only concentrations of ECMPs in solution were reported so a volume of 50ul/cm2 was used for calculations, based on recommendations for MatrigelTM coatings (Becton Dickinson).
3At the time of writing recombinant vitronectin was being promoted in combination with the hPSC media E8 and as such was being sold at low cost.