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. Author manuscript; available in PMC: 2014 Nov 13.
Published in final edited form as: J Autoimmun. 2013 Dec 28;52:130–138. doi: 10.1016/j.jaut.2013.12.005

Fig 1.

Fig 1

Representative hierarchal gating for T cell analysis. The following gating strategy was used to isolate T cell populations. (A) Initial gating used forward scatter width (FSC-Width) versus height (FSC-Height) plot to remove doublets. Next, events were gated through a forward scatter area (FSC-Area) versus CD14 and LIVE/DEAD dye to remove monocytes and dead cells, respectively. Subsequently, lymphocytes were gated by a FCS-Area versus SSC plot, followed by a gate on CD3+ cells. CD4 and CD8 T cell gates were drawn off of CD3+ cells. (B) Representative detection of cytokines produced by CD4 T cells (IFN-γ, TNF-α, IL-2, IL-17, IL-21) following no stimulation (upper panel) or PMA/IONO stimulation (lower panel). (C) Detection of cytokines (IFN-γ, TNF-α, IL-2) produced by CD8 T cells following no stimulation (lower panel) or PMA/IONO stimulation (upper panel).