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. 2014 Oct 30;13:147. doi: 10.1186/s12934-014-0147-7

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© Dato et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Intracellular adenine nucleotides concentrations in the lactic acid producing strains. CEN.PK m850 (grey bars) and CEN.PK m850 sam2Δ (white bars) were pre-grown in shake flasks and then transferred in new flasks in minimal medium containing 90 g/L of glucose for the lactic acid production phase. ATP, ADP and AMP were extracted and determined by HPLC, immediately before (0 h) or after the transfer in the production medium, at the indicated times (24 h and 48 h). Nucleotide concentrations are expressed per gram of dry cell weight (DCW). The mean and SD for two independent experiments is reported.