Figure 2.

Sam2p-GFP fluorescence distribution during growth in the absence and presence of lactic acid. CEN.PK 113-11C SAM2GFP cells were grown in shake flasks in minimal (YNB) medium with 2% w/v glucose without or with the addition of different concentrations of lactic acid (pH 5, pH 3, 12 g/L and 20 g/L lactic acid at pH3). Epifocal microscope images were taken at 16 and 40 hours after inoculation, corresponding to exponential growth phase and early stationary phase, respectively. Pictures show Sam2p-GFP fluorescence in the green field. White arrows indicate Sam2p-GFP foci. LA: lactic acid.